This antibody worked great for fluorescent immunostaining of perfusion-fixed mice brain slices.
Dilution used: 1:1000
For more information see Zha et al. Cell Reports 2020, “VMHvl-Projecting Vglut1+ Neurons in the Posterior Amygdala Gate Territorial Aggression”. https://pubmed.ncbi.nlm.nih.gov/32320666/
Western blot
Not Rated
Immunostaining
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On January 17, 2020Youngseok Lee, Kookmin University wrote:
5
This antibody works excellent for immunohistochemistry.
Dilution used: 1:1000
This antibody worked fine for immunostaining of perfusion-fixed mice tissue for fluorescence microscopy.
Dilution used: 1:1000
For more information see Boyle et al., Defining a Spinal Microcircuit that Gates Myelinated Afferent Input: Implications for Tactile Allodynia, Cell Reports, vol 28, issue 2, p526-540, July 9, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30806-X).
On June 18, 2020 Bing Zhang initiative wrote:
Great for ICC on fly brain. Dilution: 1:1000
On June 16, 2020 Bing Zhang initiative wrote:
Great for ICC on fly brain. Dilution: 1:250
On May 25, 2020 Xi Zha wrote:
This antibody worked great for fluorescent immunostaining of perfusion-fixed mice brain slices.
Dilution used: 1:1000
For more information see Zha et al. Cell Reports 2020, “VMHvl-Projecting Vglut1+ Neurons in the Posterior Amygdala Gate Territorial Aggression”.
https://pubmed.ncbi.nlm.nih.gov/32320666/
On January 17, 2020 Youngseok Lee, Kookmin University wrote:
This antibody works excellent for immunohistochemistry.
Dilution used: 1:1000
For more information see Rimal et al. Cell Reports 2019, Mechanism of Acetic Acid Gustatory Repulsion in Drosophila
https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30060-9#secsectitle0020
On October 17, 2019 David I. Hughes, University of Glasgow wrote:
This antibody worked fine for immunostaining of perfusion-fixed mice tissue for fluorescence microscopy.
Dilution used: 1:1000
For more information see Boyle et al., Defining a Spinal Microcircuit that Gates Myelinated Afferent Input: Implications for Tactile Allodynia, Cell Reports, vol 28, issue 2, p526-540, July 9, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30806-X).
Hughes Laboratory:
https://specciebear.wixsite.com/spinal-cord-hughes