OTUB1, rabbit mAb clone D8F7, Cell Signaling, 3783

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One User Review

  • This antibody works excellent for detection of OTUB1 in murine hippocampal tissue. The tissue was lysed and sonicated in lysis buffer listed below.
    Dilution 1:1000

    Lysis buffer:

    • 20 mM MOPS
    • 2 mM EGTA (to bind calcium);
    • 5 mM EDTA (to bind magnesium and manganese);
    • 50 mM sodium fluoride (to inhibit protein-serine phosphatases);
    • 60 mM β-glycerophosphate, pH 7.2 (to inhibit protein-serine phosphatases);
    • 25 mM sodium pyrophosphate (to inhibit protein-serine phosphatases);
    • 2.5 mM sodium orthovanadate (to inhibit protein-tyrosine phosphatases);
    • 50nM phenylarsine oxide (to inhibit protein-tyrosine phosphatases);
    • 1% Triton X-100
    • 0.05% sodium dodecylsulphate (SDS)
    cOmplete™ Protease Inhibitor and DTT added before use.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Flow cytometry