Tested with IHC on DRG sections from naïve mice either fixation perfused with 4% PFA or perfused with PBS. At our hands, the antibody was found to work best at 1:500-1:1000 for IHC and works good with antigen retrieval (microwaved in citrate buffer) The antibody stained with apparent specificity toward smaller diameter neurons and no nuclear staining was observed.
On September 24, 2019 Ole Ahlgreen wrote:
Tested with IHC on DRG sections from naïve mice either fixation perfused with 4% PFA or perfused with PBS. At our hands, the antibody was found to work best at 1:500-1:1000 for IHC and works good with antigen retrieval (microwaved in citrate buffer) The antibody stained with apparent specificity toward smaller diameter neurons and no nuclear staining was observed.