CD163, murine mAb clone MAC2-158, R-PE conjugated, IQProducts, IQP-570R

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One User Review

  • Used on (frozen) human PBMCs in a 10 color flow panel on LRS-Fortessa.

    Recommended use: 10µL/100µL. Titrated as single stain using 1X, 2X, 8X and 10X and found best stain index using 10X = 1µL.

    Can easily distinguish +/- populations and measure the MFI.

    Samples are blocked using 10% human AB serum and stained fifty/fifty in PBS with 1% BSA and Brilliant Stain Buffer (BD horizon), and fixed in 0.9% formaldehyde. Compensated on OneComp eBeads (Thermofisher Scientific)

    Western blotNot Rated
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometry55555
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM