For the detection of neurons and especially their processes guinea pig-anti-βIII-tubulin (Synaptic Systems, Göttingen, Germany; 302304) might be considered as very useful antiserum. Applying indirect fluorescence staining with green fluorescent carbocyanine (Cy)2-tagged donkey-anti-guinea pig IgG this immunoreagent reveals fine morphological details in paraformaldehyde frozen sections of rodent brains. Recently, it was also used to demonstrate alterations resulting after experimentally induced stroke in mice (Aleithe et al., Mol Neurobiol, 2019, Fig. 3).
This antibody is an outstanding immunohistochemical marker
On July 6, 2020 Jamie Rose, University of Edinburgh wrote:
Used for IF. Never works. Does not work on samples confirmed MAP2 positive by SYSY188004.
For more information see Pickett et al. Cell Reports 2019, “Amyloid Beta and Tau Cooperate to Cause Reversible Behavioral and Transcriptional Deficits in a Model of Alzheimer’s Disease”.
https://www.sciencedirect.com/science/article/pii/S2211124719315268?dgcid=rss_sd_all#!
On August 15, 2019 Wolfgang Härtig, University of Leipzig wrote:
For the detection of neurons and especially their processes guinea pig-anti-βIII-tubulin (Synaptic Systems, Göttingen, Germany; 302304) might be considered as very useful antiserum. Applying indirect fluorescence staining with green fluorescent carbocyanine (Cy)2-tagged donkey-anti-guinea pig IgG this immunoreagent reveals fine morphological details in paraformaldehyde frozen sections of rodent brains. Recently, it was also used to demonstrate alterations resulting after experimentally induced stroke in mice (Aleithe et al., Mol Neurobiol, 2019, Fig. 3).
This antibody is an outstanding immunohistochemical marker
Aleithe et al., Mol Neurobiol, 2019: https://link.springer.com/article/10.1007/s12035-019-1604-4