Related topics: Alphabetical list, Cancer, Signaling cascades
See list of additional Phospho-HER2/ErbB2 (Tyr1248) antibodies at Linscott’s Directory
On July 4, 2019 Anders Dalby wrote:
This antibody did not work very well for me. Little to no signal was observed at ~190kDa marker in wildtype cortical neurons from mice.
Dilution 1:1000
Lysis buffer:
• 20 mM MOPS • 2 mM EGTA (to bind calcium); • 5 mM EDTA (to bind magnesium and manganese); • 50 mM sodium fluoride (to inhibit protein-serine phosphatases); • 60 mM β-glycerophosphate, pH 7.2 (to inhibit protein-serine phosphatases); • 25 mM sodium pyrophosphate (to inhibit protein-serine phosphatases); • 2.5 mM sodium orthovanadate (to inhibit protein-tyrosine phosphatases); • 50nM phenylarsine oxide (to inhibit protein-tyrosine phosphatases); • 1% Triton X-100 • 0.05% sodium dodecylsulphate (SDS) cOmplete™ Protease Inhibitor and DTT added before use.
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On July 4, 2019 Anders Dalby wrote:
This antibody did not work very well for me. Little to no signal was observed at ~190kDa marker in wildtype cortical neurons from mice.
Dilution 1:1000
Lysis buffer:
• 20 mM MOPS
• 2 mM EGTA (to bind calcium);
• 5 mM EDTA (to bind magnesium and manganese);
• 50 mM sodium fluoride (to inhibit protein-serine phosphatases);
• 60 mM β-glycerophosphate, pH 7.2 (to inhibit protein-serine phosphatases);
• 25 mM sodium pyrophosphate (to inhibit protein-serine phosphatases);
• 2.5 mM sodium orthovanadate (to inhibit protein-tyrosine phosphatases);
• 50nM phenylarsine oxide (to inhibit protein-tyrosine phosphatases);
• 1% Triton X-100
• 0.05% sodium dodecylsulphate (SDS)
cOmplete™ Protease Inhibitor and DTT added before use.