Related topics: Alphabetical list, Lysosome markers
See list of additional Rab-GDI 1 antibodies at Linscott’s Directory
On July 4, 2019 Anders Dalby wrote:
This antibody works excellent for detecting Rab-GDI1 in lysates from mouse hippocampal tissue. Very clear bands developed after short exposure.
Dilution 1:1000
Lysis buffer:
• 20 mM MOPS • 2 mM EGTA (to bind calcium); • 5 mM EDTA (to bind magnesium and manganese); • 50 mM sodium fluoride (to inhibit protein-serine phosphatases); • 60 mM β-glycerophosphate, pH 7.2 (to inhibit protein-serine phosphatases); • 25 mM sodium pyrophosphate (to inhibit protein-serine phosphatases); • 2.5 mM sodium orthovanadate (to inhibit protein-tyrosine phosphatases); • 50nM phenylarsine oxide (to inhibit protein-tyrosine phosphatases); • 1% Triton X-100 • 0.05% sodium dodecylsulphate (SDS) cOmplete™ Protease Inhibitor and DTT added before use.
Δ
On July 4, 2019 Anders Dalby wrote:
This antibody works excellent for detecting Rab-GDI1 in lysates from mouse hippocampal tissue. Very clear bands developed after short exposure.
Dilution 1:1000
Lysis buffer:
• 20 mM MOPS
• 2 mM EGTA (to bind calcium);
• 5 mM EDTA (to bind magnesium and manganese);
• 50 mM sodium fluoride (to inhibit protein-serine phosphatases);
• 60 mM β-glycerophosphate, pH 7.2 (to inhibit protein-serine phosphatases);
• 25 mM sodium pyrophosphate (to inhibit protein-serine phosphatases);
• 2.5 mM sodium orthovanadate (to inhibit protein-tyrosine phosphatases);
• 50nM phenylarsine oxide (to inhibit protein-tyrosine phosphatases);
• 1% Triton X-100
• 0.05% sodium dodecylsulphate (SDS)
cOmplete™ Protease Inhibitor and DTT added before use.