This antibody did not work well for me. The antibody was tested on lysates from 11DIV primary cortical neurons from mice. A very faint band was observed after a very long exposure time using ECL Prime (Amersham), however the signal was too weak to quantify.
Other antibodies were tested as well with great results (e.g. GABA(A) RECEPTOR SUBTYPE ALPHA 1, SYNAPTIC SYSTEMS # 224 203) on the same lysates.
On May 31, 2019 Anders Dalby wrote:
This antibody did not work well for me. The antibody was tested on lysates from 11DIV primary cortical neurons from mice. A very faint band was observed after a very long exposure time using ECL Prime (Amersham), however the signal was too weak to quantify.
Other antibodies were tested as well with great results (e.g. GABA(A) RECEPTOR SUBTYPE ALPHA 1, SYNAPTIC SYSTEMS # 224 203) on the same lysates.