Among several antibodies specifically recognizing the calcium-binding protein calbindin-D 28kD, rabbit-anti-calbindin (Synaptic Systems, Göttingen, Germany; 214002) shows excellent staining properties including the visualization of fine processes and other subcellular details even after conventional indirect immunofluorescence labelling. Thereby, it is also useful in combination with guinea pig-anti-parvalbumin (and highly-purified carbocyanine-tagged secondary antibodies detecting either rabbit IgG or guinea pig IgG selectively). The co-expression of both calcium-binding proteins in several neurons of the mouse forebrain after ischemia and in non-affected tissue was recently reported (Härtig et al 2016 Neuroscience – Fig 8). Additionally, the same paper revealed the allocation of calbindin and glutamate decarboxylase (GAD) as the marker enzyme for GABAergic neurons (Fig 9).
On January 27, 2019 Wolfgang Härtig, University of Leipzig wrote:
Among several antibodies specifically recognizing the calcium-binding protein calbindin-D 28kD, rabbit-anti-calbindin (Synaptic Systems, Göttingen, Germany; 214002) shows excellent staining properties including the visualization of fine processes and other subcellular details even after conventional indirect immunofluorescence labelling. Thereby, it is also useful in combination with guinea pig-anti-parvalbumin (and highly-purified carbocyanine-tagged secondary antibodies detecting either rabbit IgG or guinea pig IgG selectively). The co-expression of both calcium-binding proteins in several neurons of the mouse forebrain after ischemia and in non-affected tissue was recently reported (Härtig et al 2016 Neuroscience – Fig 8). Additionally, the same paper revealed the allocation of calbindin and glutamate decarboxylase (GAD) as the marker enzyme for GABAergic neurons (Fig 9).
I can highly recommend this antibody for IHC
Härtig et al. 2016 Neuroscience: https://www.sciencedirect.com/science/article/abs/pii/S0306452216304420?via%3Dihub