A marker for vascular basement membranes with very low background staining is goat-anti-collagen IV from Merck Millipore (formerly Chemicon; AB749). This immunoreagent appears really helpful if the counterstaining of other markers is possible only by applying restricted host species (such as rabbit and mouse). Goat-anti-collagen IV was used for the detection of affected tissues after experimentally induced stroke in rats (Michalski et al 2017 PLoS One – Figs 8,9).
On January 13, 2019 Wolfgang Härtig, University of Leipzig wrote:
A marker for vascular basement membranes with very low background staining is goat-anti-collagen IV from Merck Millipore (formerly Chemicon; AB749). This immunoreagent appears really helpful if the counterstaining of other markers is possible only by applying restricted host species (such as rabbit and mouse). Goat-anti-collagen IV was used for the detection of affected tissues after experimentally induced stroke in rats (Michalski et al 2017 PLoS One – Figs 8,9).
Michalski et al. 2017 PLoS One: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0174996