Related topics: Alphabetical list, Alzheimer's disease, Cellular marker proteins, Neurodegeneration, Neuroscience, Neurotransmission
See list of additional CHAT antibodies at Linscott’s Directory
On May 29, 2020
Robert Lewis wrote:
This antibody works really well for immunofluorescence in paraformaldehyde-fixed mouse brain tissue. I have used dilutions from 1:600-1:1000 successfully in floating striatal tissue sections.
Lewis et al 2020: https://www.cell.com/cell-reports/fulltext/S2211-1247(20)30427-7#secsectitle0085
On January 28, 2020
Hiromi Kumamaru, University of California wrote:
This antibody works fine for immunohistochemistry.
Dilution used: 1:200
For more information see Kumamaru et al. Cell Reports 2019, “Regenerating Corticospinal Axons Innervate Phenotypically Appropriate Neurons within Neural Stem Cell Grafts”
On December 21, 2018
Wolfgang Härtig, University of Leipzig wrote:
By applying this primary antibody to immunofluorescence labeling you might expect very good results in paraformaldehyde-fixed tissues.
Goat-anti-choline acetyltransferase, affinity-purified) from Merck Millipore (formerly Chemicon; AB144P) applied to forebrain sections from various mammalian species including mouse (Härtig et al 2014, Neuropathol Appl Neurobiol, Fig 2), hamster (Härtig et al 2007 Eur J Neurosci – Figs 4,5 ) and rat (Kacza et al 2000 Brain Res, Fig 3).
Härtig et al. 2014: https://onlinelibrary.wiley.com/doi/full/10.1111/nan.12050
Härtig et al. 2007: https://onlinelibrary.wiley.com/doi/full/10.1111/j.1460-9568.2006.05250.x
Kacza et al. 2000: https://www.sciencedirect.com/science/article/pii/S0006899300022393?via%3Dihub