On June 30, 2020Nicola Micali, Yale University wrote:
5
Great antibody for IF. Concentration indicated by manufacturer’s protocol.
For more information see Micali et al. Cell Reports 2020, “Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates”. https://www.sciencedirect.com/science/article/pii/S2211124720305489
Works roughly for ICC of hiPSC derived neuronal cultures.
Dilution used: 1:200
For more information see Ghatak et al. eLife 2019, “Mechanisms of hyperexcitability in Alzheimer’s disease hiPSC-derived neurons and cerebral organoids vs. isogenic controls“ https://elifesciences.org/articles/50333v1
On June 30, 2020 Nicola Micali, Yale University wrote:
Great antibody for IF. Concentration indicated by manufacturer’s protocol.
For more information see Micali et al. Cell Reports 2020, “Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates”.
https://www.sciencedirect.com/science/article/pii/S2211124720305489
On May 18, 2020 Swagata Ghatak, The Scripps Research Institute wrote:
Works roughly for ICC of hiPSC derived neuronal cultures.
Dilution used: 1:200
For more information see Ghatak et al. eLife 2019, “Mechanisms of hyperexcitability in Alzheimer’s disease hiPSC-derived neurons and cerebral organoids vs. isogenic controls“
https://elifesciences.org/articles/50333v1
On December 26, 2018 Suleiman Minhas, Bart De Strooper lab, KU Leuven wrote:
We used this antibody for IHC staining of 4% PFA perfused of mouse brain tissues (vibratome sections). It works very well.