Related topics: Alphabetical list, Alzheimer's disease, Cytoskeleton, Neurodegeneration, Neuroscience, Parkinson's disease
See list of additional Tau antibodies at Linscott’s Directory
On December 18, 2018
Ebru Ercan-Herbst, BioMed X Innovation Center, Heidelberg, Germany wrote:
This antibody was included in a study testing 49 commercial available Tau antibodies including both pan-Tau antibodies and antibodies specifically recognizing Tau with post-translational modifications (PTM).
The antibodies were tested by peptide array to assess PTM specificity and by immuno-blotting of mouse brain lysates obtained from hTg-Tau (expressing all six human isoforms of Tau) and Tau knock out mice to test Tau specificity. A subset of 35 antibodies was subsequently tested by immunofluorescence staining of human iPSC derived neurons (see reference below for details).
WB: 1/1000, Very good – weak but specific signal for tau
The study was published in Ercan et al. Molecular Neurodegeneration (2017) 12:87 “A validated antibody panel for the characterization of tau post-translational modifications” and demonstrates that commercially available antibodies show a significant lack of specificity. In particular, PTM-specific antibodies very often recognize non-modified version of Tau.