We have used this antibody for WB detection of pERK in the scleractinian coral Stylophora pistillata. Samples were protein extracts from coral nubbins incubated in the absence (Control) or presence of the MEK inhibitor U0126. A very nice signal was obtained with 1 µg antibody/ml, and the antibody detects Stylophora pistillata pERK equally well as pERK in lysates obtained from human fibroblasts.
For further details see Courtial et al., 2017 “Validation of commercial ERK antibodies against the ERK orthologue of the scleractinian coral Stylophora pistillata” (https://f1000research.com/articles/6-577/v2)
On August 26, 2018 Vincent Picco, Département de Recherche en Biologie Médicale, Centre Scientifique de Monaco wrote:
We have used this antibody for WB detection of pERK in the scleractinian coral Stylophora pistillata. Samples were protein extracts from coral nubbins incubated in the absence (Control) or presence of the MEK inhibitor U0126. A very nice signal was obtained with 1 µg antibody/ml, and the antibody detects Stylophora pistillata pERK equally well as pERK in lysates obtained from human fibroblasts.
For further details see Courtial et al., 2017 “Validation of commercial ERK antibodies against the ERK orthologue of the scleractinian coral Stylophora pistillata” (https://f1000research.com/articles/6-577/v2)