Recommended 20 ul per million cells. Used 10ul for 300,000 cells (PBMCs).
Strong signal, optimal for staining of highly expressed extracellular markers.
Western blot
Not Rated
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Luminex
Not Rated
EM
Not Rated
On August 20, 2018Kasper Mølgaard Jensen wrote:
4
Used to perform single stainings of GFP positive CAR T cells to determine basic phenotype (CD3, CD4, CD8) before and after sorting using CD4/CD8 beads or flow cytometry cell sorting. Cells were stained in FACS tubes. No antibody titration or compensation were performed.
Working solution of 20uL in 200uL sample to stain approx. 500.000 cells.
On January 9, 2019 Teresa Martín, KU wrote:
Recommended 20 ul per million cells. Used 10ul for 300,000 cells (PBMCs).
Strong signal, optimal for staining of highly expressed extracellular markers.
On August 20, 2018 Kasper Mølgaard Jensen wrote:
Used to perform single stainings of GFP positive CAR T cells to determine basic phenotype (CD3, CD4, CD8) before and after sorting using CD4/CD8 beads or flow cytometry cell sorting. Cells were stained in FACS tubes. No antibody titration or compensation were performed.
Working solution of 20uL in 200uL sample to stain approx. 500.000 cells.