Used to stain cells for the intracellular expression of CD107a in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in 96-well plate during assay incubation in combination with a protein transport inhibitor (Golgi Plug). Antibody titrations and compensation were previously performed in-lab.
0,3uL mixed in a GolgiPlud mastermix. mastermix added to each well in a 96-well plate for the duration of assay incubation at 37C (5-7h)
Western blot
Not Rated
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Luminex
Not Rated
EM
Not Rated
On August 20, 2018Kasper Mølgaard Jensen wrote:
4
Used to stain cells for the intracellular expression of CD107a in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in 96-well plate during assay incubation in combination with a protein transport inhibitor (Golgi Plug). Antibody titrations and compensation were previously performed in-lab.
Working solution of 0,3uL in 20uL mastermix to stain approx. 300.000 cells.
On August 21, 2018 Arianna Draghi wrote:
Used to stain cells for the intracellular expression of CD107a in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in 96-well plate during assay incubation in combination with a protein transport inhibitor (Golgi Plug). Antibody titrations and compensation were previously performed in-lab.
0,3uL mixed in a GolgiPlud mastermix. mastermix added to each well in a 96-well plate for the duration of assay incubation at 37C (5-7h)
On August 20, 2018 Kasper Mølgaard Jensen wrote:
Used to stain cells for the intracellular expression of CD107a in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in 96-well plate during assay incubation in combination with a protein transport inhibitor (Golgi Plug). Antibody titrations and compensation were previously performed in-lab.
Working solution of 0,3uL in 20uL mastermix to stain approx. 300.000 cells.