Used to stain cells for the intracellular expression of cytokines in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in FACS tubes after assay incubation and after permeabilization. Antibody titrations and compensation were previously performed in-lab.
a total of 1,0uL used in a 20uL mastermix with anti-IFN used to stain approx. 300.000 cells.
Western blot
Not Rated
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Luminex
Not Rated
EM
Not Rated
On August 20, 2018Kasper Mølgaard Jensen wrote:
4
Used to stain cells for the intracellular expression of cytokines in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in FACS tubes after assay incubation and after permeabilization. Antibody titrations and compensation were previously performed in-lab.
Working solution of 1uL in 20uL mastermix to stain approx. 300.000 cells.
On August 21, 2018 Arianna Draghi wrote:
Used to stain cells for the intracellular expression of cytokines in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in FACS tubes after assay incubation and after permeabilization. Antibody titrations and compensation were previously performed in-lab.
a total of 1,0uL used in a 20uL mastermix with anti-IFN used to stain approx. 300.000 cells.
On August 20, 2018 Kasper Mølgaard Jensen wrote:
Used to stain cells for the intracellular expression of cytokines in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in FACS tubes after assay incubation and after permeabilization. Antibody titrations and compensation were previously performed in-lab.
Working solution of 1uL in 20uL mastermix to stain approx. 300.000 cells.