CD8 (human), mouse mAb (clone RPA-T8), BV421 conjugated, BD Biosciences 562428

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  • Used to analyze the phenotype of tumor infiltrating lymphocytes (TILs) before and/or after rapid expansion. Antibody mixed in mastermix to stain cells in ‘reference’ tube (CD3, CD4, CD8, Live/dead marker). Antibody titrations and compensation were previously performed in-lab.
    Working solution of 0,2uL in 20uL mastermix to stain approx. 750.000 cells in a final volume of 100uL

    Western blotNot Rated
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometry55555
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM