In “Commercially available antibodies directed against α-adrenergic receptor subtypes and other G protein-coupled receptors with acceptable selectivity in flow cytometry experiments”, Tripathi et al study the specificity of this antibody against vasopressin receptor 1A (AVPR1A). The specificity of the antibodies was tested by flow cytometry using human vascular smooth muscle cell. Here, a fluorescent signal was measured when treated with siRNA specific for AVPR1A (Human AVPR1A (552) (E-003631-00-0050)) and compared with the fluorescent signal from cells treated with non-targeting RNA.
When using this antibody, a 91% reduction in fluorescence was observed.
On October 3, 2018 Simon Molgaard wrote:
In “Commercially available antibodies directed against α-adrenergic receptor subtypes and other G protein-coupled receptors with acceptable selectivity in flow cytometry experiments”, Tripathi et al study the specificity of this antibody against vasopressin receptor 1A (AVPR1A). The specificity of the antibodies was tested by flow cytometry using human vascular smooth muscle cell. Here, a fluorescent signal was measured when treated with siRNA specific for AVPR1A (Human AVPR1A (552) (E-003631-00-0050)) and compared with the fluorescent signal from cells treated with non-targeting RNA.
When using this antibody, a 91% reduction in fluorescence was observed.
See full description in the article:
https://www.ncbi.nlm.nih.gov/pubmed/26660071