In “Commercially available antibodies directed against α-adrenergic receptor subtypes and other G protein-coupled receptors with acceptable selectivity in flow cytometry experiments”, Tripathi et al study the specificity of 8 different antibodies against α-adrenergic receptor (AR) subtypes. The specificity of the antibodies was tested by flow cytometry using human vascular smooth muscle cell. Here, a fluorescent signal was measured when treated with siRNA specific for the α-adrenergic receptor (Human ADRA2C (152) (E-005424-00-0050)) and compared with the fluorescent signal from cells treated with non-targeting RNA.
When using this antibody, a 70% reduction in fluorescence was observed.
On October 3, 2018 Simon Molgaard wrote:
In “Commercially available antibodies directed against α-adrenergic receptor subtypes and other G protein-coupled receptors with acceptable selectivity in flow cytometry experiments”, Tripathi et al study the specificity of 8 different antibodies against α-adrenergic receptor (AR) subtypes. The specificity of the antibodies was tested by flow cytometry using human vascular smooth muscle cell. Here, a fluorescent signal was measured when treated with siRNA specific for the α-adrenergic receptor (Human ADRA2C (152) (E-005424-00-0050)) and compared with the fluorescent signal from cells treated with non-targeting RNA.
When using this antibody, a 70% reduction in fluorescence was observed.
See full description in the article:
https://www.ncbi.nlm.nih.gov/pubmed/26660071