Jositsch and colleagues from Justus-Liebig-University Giessen reported in 2009 an extensive study, in which they evaluate 24 muscarinic acetylcholine receptor antibodies including 16 commercially available.
Antibodies directed against the five identified subtypes of muscarinic receptor (M1R-M5R) were tested in immunohistochemical labelling of wild type mouse tissue, and staining was compared to labelling of control tissue obtained from the following MR gene-deficient (-/-) mice: M1R-/-, M2R-/-, M3R-/-, M4R-/-, M5R-/- and M2/3R-/-.
The researchers found that this antibody produced identical immunohistochemical staining of wildtype and control tissue.
For more information see:
Jositsch et al. 2009, Suitability of muscarinic acetylcholine receptor antibodies for immunohistochemistry evaluated on tissue sections of receptor gene-deficient mice https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3896859/
On July 3, 2018 Camilla Gustafsen, Aarhus Universitet wrote:
Jositsch and colleagues from Justus-Liebig-University Giessen reported in 2009 an extensive study, in which they evaluate 24 muscarinic acetylcholine receptor antibodies including 16 commercially available.
Antibodies directed against the five identified subtypes of muscarinic receptor (M1R-M5R) were tested in immunohistochemical labelling of wild type mouse tissue, and staining was compared to labelling of control tissue obtained from the following MR gene-deficient (-/-) mice: M1R-/-, M2R-/-, M3R-/-, M4R-/-, M5R-/- and M2/3R-/-.
The researchers found that this antibody produced identical immunohistochemical staining of wildtype and control tissue.
For more information see:
Jositsch et al. 2009, Suitability of muscarinic acetylcholine receptor antibodies for immunohistochemistry evaluated on tissue sections of receptor gene-deficient mice
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3896859/