Sean et al., Wirchows Archiv describe the validation of eight commercial antibodies raised against ROR2. This commonly used antibody was one of three antibodies tested by western blots on colorectal cancer cell lines (used 1:1000). Here, this antibody was the only one of the three tested that detected a band at the correct size (105kDa). Furthermore, no bands were detected in the cells lacking expression.
When staining cells overexpressing ROR2, the antibody was able to detect the expression when used at 1:70. Similarly, the antibody also worked for staining tissue samples from patients suffering from colorectal cancer (1:70). See full description in the article.
On April 23, 2018 Caroline Ford wrote:
Sean et al., Wirchows Archiv describe the validation of eight commercial antibodies raised against ROR2. This commonly used antibody was one of three antibodies tested by western blots on colorectal cancer cell lines (used 1:1000). Here, this antibody was the only one of the three tested that detected a band at the correct size (105kDa). Furthermore, no bands were detected in the cells lacking expression.
When staining cells overexpressing ROR2, the antibody was able to detect the expression when used at 1:70. Similarly, the antibody also worked for staining tissue samples from patients suffering from colorectal cancer (1:70). See full description in the article.