This antibody was included in a test of ten commercially available antibodies against alpha-1-adrenergic receptor subtypes. All antibodies were tested using Western blotting of brain and heart tissue from WT mice and knock out controls with genetic deletion of one, two or all three alpha-1-adrenergic receptor subtypes.
None of the antibodies detected a band of the appropriate size, or a band of larger or smaller size, that was present in WT mouse heart or brain and absent in KO heart or brain.
On April 15, 2018 Paul Simpson, UCSF wrote:
This antibody was included in a test of ten commercially available antibodies against alpha-1-adrenergic receptor subtypes. All antibodies were tested using Western blotting of brain and heart tissue from WT mice and knock out controls with genetic deletion of one, two or all three alpha-1-adrenergic receptor subtypes.
None of the antibodies detected a band of the appropriate size, or a band of larger or smaller size, that was present in WT mouse heart or brain and absent in KO heart or brain.
For further information see BC Jensen et al., 2009 (https://link.springer.com/article/10.1007%2Fs00210-008-0368-6)