2 User Reviews

  • We have used this GFRa1 antibody for IHC on PFA-fixed cryosections of mouse brain treated with antigen retrieval kit.

    In the hippocampus, we see a similar subcellular distribution as in cultured hippocamal neurons.

    Upon preincubation of the antibody with purified GFRa1 the signal was substantially reduced.

    Western blotNot Rated
    Immunostaining33333
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Really great antibody in all categories.

    Detects endogenous mouse GFRa1 in hippocampal neurons both by IF and WB. Great for WB and IF also of recombinant rat GFRa1 expressed in 293 cells as shown in the image above (GFRa1 is green and nuclei are blue).
    Detects GFRa1 in brain homogenates as well.

    Excellent for IP of rat GFRa1 expressed in 293 cells.

    IF 2 ug/ml
    WB 0.1 ug/ml
    Diluted antibody can be frozen and reused for Western blotting several times.

    Western blot55555
    Immunostaining55555
    IP55555
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

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Western blot
Immunostaining
IP
ELISA
Flow cytometry
Luminex