By manufactures instruction this clone is not recommended for intracellular cytokine staining (ICS).
PE anti-CD56 was used in an antibody cocktail for ICS of PBMCs Expressing a chimeric antigen receptor.
Protocol in short: 3uL/sample added to antibody cocktail at a final volume of 20uL. 20uL antibody cocktail added to cells to a final volume of approximately 30uL. Cells incubated in dark for 20 minutes before washing, fixation, and intracellular staining.
Indeed, anti-CD56 clone MY31 did not work in ICS for identifying CD3-/CD56+ NK cells.
On October 29, 2017 Kasper Mølgaard Jensen, Center for Cancer Immune Therapy (CCIT), Herlev Hospital, Denmark wrote:
Anti-human CD56, PE-conjugated, clone MY31, BD Bioscience #345810
By manufactures instruction this clone is not recommended for intracellular cytokine staining (ICS).
PE anti-CD56 was used in an antibody cocktail for ICS of PBMCs Expressing a chimeric antigen receptor.
Protocol in short: 3uL/sample added to antibody cocktail at a final volume of 20uL. 20uL antibody cocktail added to cells to a final volume of approximately 30uL. Cells incubated in dark for 20 minutes before washing, fixation, and intracellular staining.
Indeed, anti-CD56 clone MY31 did not work in ICS for identifying CD3-/CD56+ NK cells.