Antibody used in palet containing; anti-human-CD170a (BV421), -CD3 (FITC), -CD4 (PerCP), -CD8 (QD605), -CD56 (PE), -TNFa (APC), -IFNy (PeCy7) for intracellular cytokine staining (ICS) of fixated/permeabilised T cells expressing a chimeric receptor and activated through antigen presentation.
Works very well for detection CD107a in flow cytometry. Antibody was diluted in a master mix as 0.3uL anti-CD170a antibody for 50uL total volume per sample.
On October 29, 2017 Kasper Mølgaard Jensen, Center for Cancer Immune Therapy (CCIT), Herlev Hospital, Denmark wrote:
Anti-human CD170a (BV421 conjugated), clone H4A3, BD Bioscience, #562623
Antibody used in palet containing; anti-human-CD170a (BV421), -CD3 (FITC), -CD4 (PerCP), -CD8 (QD605), -CD56 (PE), -TNFa (APC), -IFNy (PeCy7) for intracellular cytokine staining (ICS) of fixated/permeabilised T cells expressing a chimeric receptor and activated through antigen presentation.
Works very well for detection CD107a in flow cytometry. Antibody was diluted in a master mix as 0.3uL anti-CD170a antibody for 50uL total volume per sample.