Related topics: Alphabetical list, Metabolism
See list of additional GAPDH antibodies at Linscott’s Directory
On May 4, 2020
Tiago Gil Oliveira, University of Minho wrote:
I have used this antibody for western blotting of total lysates from mice hippocampal tissue in a dilution of 1:3000. This antibody works so well for me, that I often use it as a control of my blotting protocol.
For more information see Santa-Marinha et al. Cell Reports 2020, “Phospholipase D1 Ablation Disrupts Mouse Longitudinal Hippocampal Axis Organization and Functioning”
On April 23, 2020
Vishal Gohil, Texas A&M University wrote:
Used for western blotting with great results.
Dilution used: 1:1000
For more information see Soma et al. Cell Reports 2019, “COA6 Is Structurally Tuned to Function as a Thiol-Disulfide Oxidoreductase in Copper Delivery to Mitochondrial Cytochrome c Oxidase”. https://www.sciencedirect.com/science/article/pii/S2211124719315360#bib15
On November 25, 2019
Giorgio Ramadori, University of Geneva, Department of Cellular Physiology and Metabolism wrote:
We have used this antibody for western blotting with great succes.
For more information see Aras et al., 2019, Cell Reports 27, 2385–2398 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30573-X)
On May 31, 2019
Teresa Martín wrote:
Used as a primary antibody in Western Blot. Amount of cells lysated for the WB varied from 1×106 to 2.5×106. Really strong signal, can be used in lower concentrations. Incubation 1.5 hrs.
Antibody was diluted 1:1000 in TBST + BSA (1%).
On October 29, 2017
Peter Lund Ovesen wrote:
GAPDH (14C10) Rabbit mAb #2118 Cell Signaling
Excellent antibody for the loading marker GAPDH. Used for reprobing of membranes at a dilution of 1:5000.
Tested on Human neuroblastoma cell line SH-SY5Y.
Size: 37 kDA