Angiotensin Mas Receptor, rabbit pAb, Alomone Labs, AAR-013

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One User Review

  • This antibody was tested in heart and kidney mouse tissue in WB and IHC.
    In both applications, the antibody failed to detect a specific immuno-reactivity signal in WT mice compared to the signal observed in Mas receptor knock-out animals. The expression of Mas receptor in tested tissue was confirmed by RT-PCR analysis. No Mas receptor mRNA was detected in Mas receptor knock out mouse.

    In addition, the antibody was tested in HEK293 cells transiently transfected with Mas receptor (c-myc tagged). Again, no signal was detected in neither WB nor immunofluorescence experiments. Successful transfection was confirmed with anti c-myc antibody.

    The tested antibody was from lot # AN0702, and was used in the following dilutions:
    WB 1:1000
    IHC 1:100
    IF 1:100

    Ref: Burghi et al., PLoS One. 2017 Aug 16;12(8):e0183278, a table summary of antibodies tested in the study is inserted

    Western blot11111
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Flow cytometry