Related topics: Alphabetical list, Cancer biology, Cell adhesion, Extracellular matrix
See list of additional Heparanase antibodies at Linscott’s Directory
On September 11, 2017
Argyris Spyrou, Department of Immunology, Genetics and Pathology, Uppsala University wrote:
This antibody (1:2500 in blocking buffer (1x TBST with 5% w/v nonfat dry milk)) was used for Western blot of heparanse in lysates of human CNS embryonal tumors, medulloblastomas and tumor cell lines D283, D324 and PFSK-1 (ref: Spyrou et al., Mol Cancer Ther, 2017 Aug; 16(8): 1705-1716)
The antibody was provided by Dr. Robert Heinrikson (Pfizer Inc., Kalamazoo), and first described in Fairbanks et al. October 15, 1999 The Journal of Biological Chemistry 274, 29587-29590.
The 1453ab was raised against the entire 65 kDa heparanase precursor isolated from the conditioned medium of heparanase-transfected 293 cells. This antibody recognizes both the latent 65 kDa precursor and the 50kDa processed form of heparanase in immunoblotting and immunostaining (Zetser A, Levy-Adam F, Kaplan V, Gingis-Velitski S, Bashenko Y, Schubert S, et al. Processing and activation of latent heparanase occurs in lysosomes. Journal of Cell Science 2004;117(11):2249.).