Used to stain cells for phenotype gating in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in FACS tubes after assay incubation and prior to fixation and permeabilization (CD3, CD4, CD8, CD56). Antibody titrations and compensation were previously performed in-lab.
a total of 1,7uL used in a 20uL mastermix with CD3, CD8 and CD56 used to stain approx. 300.000 cells.
Western blot
Not Rated
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Luminex
Not Rated
EM
Not Rated
On September 10, 2017Kasper Mølgaard Jensen wrote:
Antibody used in palette containing; anti-human-CD170a (BV421), -CD3 (FITC), -CD4 (PerCP), -CD8 (QD605), -CD56 (PE), -TNFa (APC), -IFNy (PeCy7) for intracellular cytokine staining (ICS) of fixated/permeabilised T cells expressing a chimeric receptor and activated through antigen presentation.
Works very well for detection of lymphocytes subpopulations in flow cytometry. The antibody was diluted in a master mix as 5.0uL anti-CD4 antibody for 20uL total volume per sample.
On August 21, 2018 Arianna Draghi wrote:
Used to stain cells for phenotype gating in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in FACS tubes after assay incubation and prior to fixation and permeabilization (CD3, CD4, CD8, CD56). Antibody titrations and compensation were previously performed in-lab.
a total of 1,7uL used in a 20uL mastermix with CD3, CD8 and CD56 used to stain approx. 300.000 cells.
On September 10, 2017 Kasper Mølgaard Jensen wrote:
Anti-human CD4 (PerCP conjugated), clone OKT4, Biolegend, #317432
Antibody used in palette containing; anti-human-CD170a (BV421), -CD3 (FITC), -CD4 (PerCP), -CD8 (QD605), -CD56 (PE), -TNFa (APC), -IFNy (PeCy7) for intracellular cytokine staining (ICS) of fixated/permeabilised T cells expressing a chimeric receptor and activated through antigen presentation.
Works very well for detection of lymphocytes subpopulations in flow cytometry. The antibody was diluted in a master mix as 5.0uL anti-CD4 antibody for 20uL total volume per sample.