Used to stain cells for the intracellular expression of cytokines in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in FACS tubes after assay incubation and after permeabilization. Antibody titrations and compensation were previously performed in-lab.
1,5uL used in a 20uL mastermix with anti-TNF used to stain approx. 300.000 cells at 4 C
Western blot
Not Rated
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Luminex
Not Rated
EM
Not Rated
On September 10, 2017Kasper Mølgaard Jensen wrote:
Antibody used in palette containing; anti-human-CD170a (BV421), -CD3 (FITC), -CD4 (PerCP), -CD8 (QD605), -CD56 (PE), -TNFa (APC), -IFNy (PeCy7) for intracellular cytokine staining (ICS) of fixated/permeabilised T cells expressing a chimeric receptor and activated through antigen presentation.
Works very well for detection of intracellular IFN-y flow cytometry. The antibody was diluted in a master mix as 1.5uL anti-IFNy antibody for 20uL total volume per sample.
On August 21, 2018 Arianna Draghi wrote:
Used to stain cells for the intracellular expression of cytokines in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in FACS tubes after assay incubation and after permeabilization. Antibody titrations and compensation were previously performed in-lab.
1,5uL used in a 20uL mastermix with anti-TNF used to stain approx. 300.000 cells at 4 C
On September 10, 2017 Kasper Mølgaard Jensen wrote:
Anti-human IFN-y (Pe-Cy7 conjugated), clone B27, BD Bioscience, #557643
Antibody used in palette containing; anti-human-CD170a (BV421), -CD3 (FITC), -CD4 (PerCP), -CD8 (QD605), -CD56 (PE), -TNFa (APC), -IFNy (PeCy7) for intracellular cytokine staining (ICS) of fixated/permeabilised T cells expressing a chimeric receptor and activated through antigen presentation.
Works very well for detection of intracellular IFN-y flow cytometry. The antibody was diluted in a master mix as 1.5uL anti-IFNy antibody for 20uL total volume per sample.