Andersson et al. Nature Communications 2017 describe the validation of thirteen commercial and academic antibodies raised against estrogen receptor beta (ERbeta).
The present antibody was in house produced and failed in the IHC validation step (1:900 dilution) since it recognized both ERbeta positive cells and ERbeta negative cells.
See link to full article above.
On August 2, 2017 Cecilia Williams, KTH Royal Institute of Technology, Sweden wrote:
Andersson et al. Nature Communications 2017 describe the validation of thirteen commercial and academic antibodies raised against estrogen receptor beta (ERbeta).
The present antibody was in house produced and failed in the IHC validation step (1:900 dilution) since it recognized both ERbeta positive cells and ERbeta negative cells.
See link to full article above.