Related topics: Alphabetical list, Immunology
On June 29, 2017
Kasper Mølgaard Jensen wrote:
Biotinylated anti-human IFN-gamma mAb, clone: 7-B6-1, MABTECH, #3420-6
mAb used as detection antibody in ELISpot assay to detect cellular immune response of chimeric antigen receptor modified T-cells against CD19 positive B-cells.
Biotinylated anti-hIFNy was diluted in PBS supplemented with 1% BSA and 0.02% NaN3 to a final concentration of [0.75 ug/mL] (final volume: 75uL) (incubation two hours at RT)
Plate was washed between each step using 6 x 200uL PBS (or PBS + 0.05% tween).
Very good results were obtained with clear individual spots, a clear dose-dependent response and low background. Positive control: staphylococcal enterotoxin B [2ug/mL].