On June 29, 2017Veerle Paternoster, Aarhus Universitet wrote:
4
Histone H4K20me2, rabbit, abcam #ab9052
Tested on dilution of histone extract (acid extraction, mouse brain) and cell lysates (RIPA buffer, mouse brain).
2 series of bands, but one with approriate MW.
WB details: BIS-TRIS gel+XT MES running buffer, blocked in TBS+5%BSA, 1:2000 dilution (picture inserted)
On June 29, 2017 Veerle Paternoster, Aarhus Universitet wrote:
Histone H4K20me2, rabbit, abcam #ab9052
Tested on dilution of histone extract (acid extraction, mouse brain) and cell lysates (RIPA buffer, mouse brain).
2 series of bands, but one with approriate MW.
WB details: BIS-TRIS gel+XT MES running buffer, blocked in TBS+5%BSA, 1:2000 dilution (picture inserted)