This antibody is excellent in all categories.
It truly has been a pleasure working with it:-)
It recognizes recombinant GDNF binding to 293 cells expressing GDNF receptors or to primary hippocampal neurons. It also appears to recognize the low endogenous GDNF levels produced by primary CNS glia in immunofluorescense stainings.
Cells have been fixed 20 min with 4% PFA.
It is also nicely applicable for IP and for recognizing recombinant GDNF in Western blotting.
It also recognizes a band in Western blotting of the predicted size in brain homogenates. The highest band intensity is observed in striatal homogenate – the striatum is site with the highest expression of GDNF.
The image above shows human GDNF (green) bound to the surface of HEK293 cells transfected with the GDNF receptor GFRa1. Cells are also stained for Lamp-1 (red) using mAb H4A3.
IF dilution: 2 ug/ml
WB dilution: 0.1 ug/ml
Diluted antibody can be frozen and reused several times for Western blotting.
On February 14, 2012 Simon Glerup, Aarhus wrote:
This antibody is excellent in all categories.
It truly has been a pleasure working with it:-)
It recognizes recombinant GDNF binding to 293 cells expressing GDNF receptors or to primary hippocampal neurons. It also appears to recognize the low endogenous GDNF levels produced by primary CNS glia in immunofluorescense stainings.
Cells have been fixed 20 min with 4% PFA.
It is also nicely applicable for IP and for recognizing recombinant GDNF in Western blotting.
It also recognizes a band in Western blotting of the predicted size in brain homogenates. The highest band intensity is observed in striatal homogenate – the striatum is site with the highest expression of GDNF.
The image above shows human GDNF (green) bound to the surface of HEK293 cells transfected with the GDNF receptor GFRa1. Cells are also stained for Lamp-1 (red) using mAb H4A3.
IF dilution: 2 ug/ml
WB dilution: 0.1 ug/ml
Diluted antibody can be frozen and reused several times for Western blotting.