Affinity-purified rabbit antibodies directed against the vesicular glutamate transporters (VGLUT2; Synaptic Systems, 135403) were repeatedly found to be useful for a subpopulation of glutamatergic endings for instance in mice (Michalski et al 2013, Figs 4 and 6) and hamsters (Härtig et al 2007, Fig 7) These antibodies might be also concomitantly applied with affinity-purified antibodies recognizing VGLUT1 (also available from Synaptic Systems) to simultaneously reveal the vast majority of glutamatergic endings.
I performed IHC with this antibody on 12 um mouse olfactory bulb cryosections to stain glomerular layer. Specific staining in axon terminals but there is background.
Antibody dilution:1/500
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On January 9, 2013Simon Glerup wrote:
3
Works for Western blotting using membrane preparations of mouse hippocampus.
Dilution 1:500
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On April 16, 2012Simon Glerup, Aarhus wrote:
4
Useful antibody as a presynaptic marker in subsets of primary mouse hippocampal neurons.
On January 8, 2019 Wolfgang Härtig, University of Leipzig wrote:
I highly recommend this antibody.
Affinity-purified rabbit antibodies directed against the vesicular glutamate transporters (VGLUT2; Synaptic Systems, 135403) were repeatedly found to be useful for a subpopulation of glutamatergic endings for instance in mice (Michalski et al 2013, Figs 4 and 6) and hamsters (Härtig et al 2007, Fig 7) These antibodies might be also concomitantly applied with affinity-purified antibodies recognizing VGLUT1 (also available from Synaptic Systems) to simultaneously reveal the vast majority of glutamatergic endings.
Michalski et al. 2013: https://www.ncbi.nlm.nih.gov/pubmed/23219666
Härtig et al. 2007: https://onlinelibrary.wiley.com/doi/full/10.1111/j.1460-9568.2006.05250.x
On January 19, 2015 Hande Login wrote:
I performed IHC with this antibody on 12 um mouse olfactory bulb cryosections to stain glomerular layer. Specific staining in axon terminals but there is background.
Antibody dilution:1/500
On January 9, 2013 Simon Glerup wrote:
Works for Western blotting using membrane preparations of mouse hippocampus.
Dilution 1:500
On April 16, 2012 Simon Glerup, Aarhus wrote:
Useful antibody as a presynaptic marker in subsets of primary mouse hippocampal neurons.
IF dilution 1:100