On May 25, 2017Veerle Paternoster, Aarhus Universitet wrote:
4
Histone H4, rabbit, abcam #ab7311
Tested on dilution of histone extract (acid extraction, mouse brain) and cell lysates (RIPA buffer, mouse brain). Seems to have rather low affinity for histones in the cell extract (many aspecific bands, extremely weak band op appropriate MW). Histone extraction recommended.
WB details: BIS-TRIS gel+XT MES running buffer, blocked in TBS+5%BSA, 1:2000 dilution (picture inserted)
On May 25, 2017 Veerle Paternoster, Aarhus Universitet wrote:
Histone H4, rabbit, abcam #ab7311
Tested on dilution of histone extract (acid extraction, mouse brain) and cell lysates (RIPA buffer, mouse brain). Seems to have rather low affinity for histones in the cell extract (many aspecific bands, extremely weak band op appropriate MW). Histone extraction recommended.
WB details: BIS-TRIS gel+XT MES running buffer, blocked in TBS+5%BSA, 1:2000 dilution (picture inserted)