PSD95, guinea pAb, Synaptic Systems, 124 014

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  • Antibody: PSD95 124 014 (Synaptic Systems) – Polyclonal Guinea antibody

    DId not work very well for immunostaining mouse hippocampal neurons. PSD95 clusters was hard to distinguish from background signal.

    Neurons were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with antibody in a 1:2000 and a 1:3000 dilution in blocking buffer.

    This antibody was tested simultaneously with other antibodies for PSD95 (see below) and proved to be the second worst.
    PSD95 MAB 1596 (Chemicon/Millipore) – Mouse
    PSD95 p246 (Sigma) – Mouse (BEST)
    PSD95 MA1-046 (ThermoFisher Scientific) – Mouse

    Western blotNot Rated
    Immunostaining22222
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM