The antibody was used in IHC to stain dorsal root ganglia from rats and mice. The antibody was diluted 1:5000 (Stock concentration 5.5 mg/ml).
The staining looked good both in mouse and rat. Specific staining of satellite glial cells and very little unspecific staining in other cell types.
The dorsal root ganglia had beed fixed for 1.5 hrs (mouse) or 4 hrs (rat) in 4% PFA. Cryoprotected overnight in 30% sucrose and finally embeded in tissue tek. The sections were cut in 10 um sections.
On April 29, 2017 Sara B. Jager, Aarhus University wrote:
polyclonal rabbit anti-GS, ab49873, Abcam
The antibody was used in IHC to stain dorsal root ganglia from rats and mice. The antibody was diluted 1:5000 (Stock concentration 5.5 mg/ml).
The staining looked good both in mouse and rat. Specific staining of satellite glial cells and very little unspecific staining in other cell types.
The dorsal root ganglia had beed fixed for 1.5 hrs (mouse) or 4 hrs (rat) in 4% PFA. Cryoprotected overnight in 30% sucrose and finally embeded in tissue tek. The sections were cut in 10 um sections.