On April 29, 2017Helene H. Jensen, Aarhus University wrote:
3
Pan-Ras
Sigma (Millipore), MABS195
We used this antibody for IHC-labeling of human breast cancer tissue. We scored labeling intensity, and the antibody was useful for this purpose.
The antibody was used at 1:400 ON. The tissue was PFA-fixed, embedded in paraffin, cut at 3 um, and target retrival was performed in TEG-buffer (Tris/EGTA). The secondary antibody was used at 1:200.
On April 29, 2017 Helene H. Jensen, Aarhus University wrote:
Pan-Ras
Sigma (Millipore), MABS195
We used this antibody for IHC-labeling of human breast cancer tissue. We scored labeling intensity, and the antibody was useful for this purpose.
The antibody was used at 1:400 ON. The tissue was PFA-fixed, embedded in paraffin, cut at 3 um, and target retrival was performed in TEG-buffer (Tris/EGTA). The secondary antibody was used at 1:200.