Related topics: Alphabetical list, Cell adhesion
Zonula occludens-1 ZO-1, also known as Tight junction protein-1
See list of additional ZO-1 antibodies at Linscott’s Directory
On April 12, 2020
Wei Li, University of Chinese Academy of Sciences, Beijing wrote:
This antibody worked fine for immunostaining.
Dilution used: 1:100
For more information see Cui et al. Cell Reports 2019, “Derivation of Mouse Haploid Trophoblast Stem Cells”.
On April 29, 2017
Helene H. Jensen, Aarhus University wrote:
ZO-1 (zonula occludens 1)
We used this antibody for IHC-labeling of human breast cancer tissue. The antibody gave a specific labeling of classical tight juntion “punctae” in ducts.
The antibody was used at 1:400 ON. The tissue was PFA-fixed, embedded in paraffin, cut at 3 um, and target retrival was performed in TEG-buffer (Tris/EGTA). The secondary antibody was used at 1:200 and developed with DAB for 1 min 15 sec.