Excellent for staining pfa fixed primary hippocampal neurons.
Neurons from p0 pups were grown for 10 days in vitro before being fixed. Following fixation, the neurons were immunostained against sortilin (1:100). A clear and intense signal was observed with almost no background. Highly recommended!
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On September 11, 2017Toshiki Yabe-Wada wrote:
5
This antibody works very well for WB, IP and ELISA. It also works for indirect IF staining to see endogenous Sortilin in primary macrophages and dendritic cells.
Western blot
Immunostaining
IP
ELISA
Flow cytometry
Not Rated
Luminex
Not Rated
On October 31, 2016Andrea E. Toth, Aarhus University wrote:
5
This antibody works very well for immunofluorescence staining in rat astrocytes and brain endothelial cells. Dilution 1:200. Fixation was done both with paraformaldehyde and methanol.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On May 22, 2015Maj Ulrichsen, Aarhus University wrote:
3
This antibody works fine to detect endogenous levels of sortilin in mouse dorsal root ganglia and sciatic nerve homogenates (50 ug total protein), but it is not very sensitive for detection of sortilin in primary rat Schwann cell lysates (100 ug total protein) . Dilution 1:1000.
The antibody was used for double immunofluorescence stainings on cryo-sections of mouse brain, and with a concentration of 0.22 ug/mL (1:9000) it is still possible to get a nice staining with low background.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On December 15, 2014Laura Johnsen, Aarhus University wrote:
5
This antibody is excellent for IHC on dorsal root ganglia in mice.
Dilution: 1:100
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On May 30, 2014Maj Ulrichsen, Aarhus University wrote:
5
This antibody works very well for IHC of mouse spinal cord cryo-sections and dorsal root ganglia. I have also used it for ICC of primary dorsal root ganglia neurons. The immunofluorescence staining is vesicular and looks very specific. Dilution: 2 ug/mL.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On February 19, 2012Simon Glerup, Aarhus University wrote:
5
This antibody is great for immunohistochemistry and immunocytochemistry on mouse cryosections and primary neurons, respectively. Dilution 2 ug/ml. See image above for sortilin (green) in a DIV12 hippocampal neuron.
It is also in our hands the best antibody for immunoprecipitation of endogenous mouse sortilin.
On November 15, 2017 Simon Mølgaard wrote:
Excellent for staining pfa fixed primary hippocampal neurons.
Neurons from p0 pups were grown for 10 days in vitro before being fixed. Following fixation, the neurons were immunostained against sortilin (1:100). A clear and intense signal was observed with almost no background. Highly recommended!
On September 11, 2017 Toshiki Yabe-Wada wrote:
This antibody works very well for WB, IP and ELISA. It also works for indirect IF staining to see endogenous Sortilin in primary macrophages and dendritic cells.
On October 31, 2016 Andrea E. Toth, Aarhus University wrote:
This antibody works very well for immunofluorescence staining in rat astrocytes and brain endothelial cells. Dilution 1:200. Fixation was done both with paraformaldehyde and methanol.
On May 22, 2015 Maj Ulrichsen, Aarhus University wrote:
This antibody works fine to detect endogenous levels of sortilin in mouse dorsal root ganglia and sciatic nerve homogenates (50 ug total protein), but it is not very sensitive for detection of sortilin in primary rat Schwann cell lysates (100 ug total protein) . Dilution 1:1000.
On March 17, 2015 Pernille Thomasen, Aarhus University wrote:
The antibody was used for double immunofluorescence stainings on cryo-sections of mouse brain, and with a concentration of 0.22 ug/mL (1:9000) it is still possible to get a nice staining with low background.
On December 15, 2014 Laura Johnsen, Aarhus University wrote:
This antibody is excellent for IHC on dorsal root ganglia in mice.
Dilution: 1:100
On May 30, 2014 Maj Ulrichsen, Aarhus University wrote:
This antibody works very well for IHC of mouse spinal cord cryo-sections and dorsal root ganglia. I have also used it for ICC of primary dorsal root ganglia neurons. The immunofluorescence staining is vesicular and looks very specific. Dilution: 2 ug/mL.
On February 19, 2012 Simon Glerup, Aarhus University wrote:
This antibody is great for immunohistochemistry and immunocytochemistry on mouse cryosections and primary neurons, respectively. Dilution 2 ug/ml. See image above for sortilin (green) in a DIV12 hippocampal neuron.
It is also in our hands the best antibody for immunoprecipitation of endogenous mouse sortilin.