Related topics: RNA-binding proteins
See list of additional Rbm7 antibodies at Linscott’s Directory
On January 30, 2017
Will Garland (THJ lab) Aarhus University wrote:
Anti- Rbm7 antibody
Sigma Prestige HPA013993, Rabbit polyclonal
Used in western blot against mouse Rbm7 in whole cell extract from E14 murine embryonic stem cells.
Primary antibody was diluted 1:500 in 5% milk/PBS-T, incubated for 16 hours/overnight at 4°C
Secondary antibody; 1:5000 dilution of goat anti-rabbit/HRP in 5% milk/PBS-T (Vector), incubated for 1 hour at RT
Gel system; 10% Tris-Tricine gel
Protein was detected ~ 30 kDa
The antibody also detects a non-specific band between 35-40 kDa. This band is still present in Rbm7 knockout mES cells. It is best to use a Tricine based gel system to resolve the correct band from the non-specific band.
The antibody also detects many larger proteins >50 kDa. It is advised to cut the membrane at this point.