Related topics: DNA/RNA binding proteins, Translational regulation
See list of additional NCBP1 antibodies at Linscott’s Directory
On January 30, 2017
Will Garland (THJ lab) Aarhus University wrote:
Abcam, ab42389, Rabbit polyclonal
Used in western blot against mouse Ncbp1 in whole cell extract from E14 murine embryonic stem cells.
Primary antibody was diluted 1:5000 in 5% milk/PBS-T, incubated for 1 hour at RT
Secondary antibody; 1:5000 dilution of goat anti-rabbit/HRP in 5% milk/PBS-T (Vector), incubated for 1 hour at RT
Gel system; 8% Tris-glycine gel
Protein was detected ~ 80 kDa
Very clean blot with no additional bands.