Related topics: Neuroscience
See list of additional EXOSC3 antibodies at Linscott’s Directory
On January 30, 2017
Will Garland (THJ lab) Aarhus University wrote:
Santa Cruz, sc-98776, Rabbit polyclonal
Used in western blot against mouse Exosc3 in whole cell extract from E14 murine embryonic stem cells.
Primary antibody was diluted 1:200 in 5% milk/PBS-T, incubated for 1 hour at RT
Secondary antibody; 1:5000 dilution of goat anti-rabbit/HRP in 5% milk/PBS-T (Vector), incubated for 1 hour at RT
Gel system; 12% Tris-glycine gel
Protein was detected ~ 30 kDa
Non specific proteins were detected > 50 kDa. It is best to cut the membrane at this point.