After several trials and using different concentrations I only got negative stainings and were unable to visualize intraepidermal nerve fibers on human skin biopsies. The stainings were done on frozen thick sections, 50 micrometer in thickness and the staining performed free floating.
However, the PGP antibody CL7756AP Anti-Human PGP 9.5 (UCHL1) (184) from the same company worked very well – see other review for that antibody.
On January 30, 2017 Pall Karlson, Department of Clinical Medicine, Aarhus University wrote:
PGP 9.5 antibody
Cedarlane, CL7755AP Anti-Human PGP 9.5 (UCHL1) (199).
After several trials and using different concentrations I only got negative stainings and were unable to visualize intraepidermal nerve fibers on human skin biopsies. The stainings were done on frozen thick sections, 50 micrometer in thickness and the staining performed free floating.
However, the PGP antibody CL7756AP Anti-Human PGP 9.5 (UCHL1) (184) from the same company worked very well – see other review for that antibody.