Related topics: Cytoskeleton
See list of additional phospho-actin antibodies at Linscott’s Directory
On January 5, 2017 Veerle Paternoster, Aarhus University wrote:
Actin (Tyr53) phospho-specific antibody (rabbit polyclonal), ECM Biosciences #AP1671 Used dilution 1:1000 (recommended), blocked membrane with 5% BSA, wash steps using TBS+Tween.
Image inserted Left: actin, right: phospho specific actin-T53. 2 weak bands of appropriate size appear in HEK293 cell extract. No bands of appropriate size in mouse brain extracts (different preparation protocols).
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On January 5, 2017 Veerle Paternoster, Aarhus University wrote:
Actin (Tyr53) phospho-specific antibody (rabbit polyclonal), ECM Biosciences #AP1671
Used dilution 1:1000 (recommended), blocked membrane with 5% BSA, wash steps using TBS+Tween.
Image inserted
Left: actin, right: phospho specific actin-T53.
2 weak bands of appropriate size appear in HEK293 cell extract. No bands of appropriate size in mouse brain extracts (different preparation protocols).