On January 5, 2017Adam Munhall, Portland VA Medical Center wrote:
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Anti SUR1 (ABCC8), clone N2889/16, UC Davis/NIH Neuromab, purified, catalog 75-267, lot 449-2AK-44, against 30ug (BSA method) rat brain homogenate lysed with RIPA buffer, bloted on low autofluorescence PVDF, illuminated with anti-mouse Dylight 680 (Thermo, 1:200), canned on NIR imager, showed no bands at 1:1000 nor 1:100 dilutions. Blocking was with 3% goat or 3% BSA. Not valid for WB in rat brain.
On January 5, 2017 Adam Munhall, Portland VA Medical Center wrote:
Anti SUR1 (ABCC8), clone N2889/16, UC Davis/NIH Neuromab, purified, catalog 75-267, lot 449-2AK-44, against 30ug (BSA method) rat brain homogenate lysed with RIPA buffer, bloted on low autofluorescence PVDF, illuminated with anti-mouse Dylight 680 (Thermo, 1:200), canned on NIR imager, showed no bands at 1:1000 nor 1:100 dilutions. Blocking was with 3% goat or 3% BSA. Not valid for WB in rat brain.