On January 5, 2017Ditte Olsen, Aarhus University wrote:
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Human Syndecan-4 Antibody (R&D Systems, AF2918)
Detects faint bands in HepG2 cell lysate. The intensities are not affected by heparinase treatment of the cells prior to lysis. No bands were identified in liver homogenate from mice. I have used the antibody concentration recommended by the supplier, but the results might be improved with higher concentration.
Loaded 40 ug protein
Used 0.1 ug/ml
On January 5, 2017 Ditte Olsen, Aarhus University wrote:
Human Syndecan-4 Antibody (R&D Systems, AF2918)
Detects faint bands in HepG2 cell lysate. The intensities are not affected by heparinase treatment of the cells prior to lysis. No bands were identified in liver homogenate from mice. I have used the antibody concentration recommended by the supplier, but the results might be improved with higher concentration.
Loaded 40 ug protein
Used 0.1 ug/ml