Rabbit anti-SUV420H1 (Origene TA335571), 1:2500 in 5 ml.
Different sample preparations (all mouse brain) and different blocking agents.
A very faint band of approximate MW appears when overexposing. Lower MW isoforms are not covered by the epitope, so these must be aspecific bands.
Please find link to image above.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On January 5, 2017Veerle Paternoster, Aarhus University wrote:
2
Rabbit anti-SUV420H1 (Origene TA335571), 1:2500 in 5 ml.
Loaded: extract from HEK293T cells, mouse brain and pig brain. Several bands visible, though not at expected MW of canoncical form (99kDa). Bands may represent shorter isoforms.
WB blocked with milk, 1AB overnight incubation at 4*C.
On February 24, 2017 Veerle Paternoster wrote:
Rabbit anti-SUV420H1 (Origene TA335571), 1:2500 in 5 ml.
Different sample preparations (all mouse brain) and different blocking agents.
A very faint band of approximate MW appears when overexposing. Lower MW isoforms are not covered by the epitope, so these must be aspecific bands.
Please find link to image above.
On January 5, 2017 Veerle Paternoster, Aarhus University wrote:
Rabbit anti-SUV420H1 (Origene TA335571), 1:2500 in 5 ml.
Loaded: extract from HEK293T cells, mouse brain and pig brain. Several bands visible, though not at expected MW of canoncical form (99kDa). Bands may represent shorter isoforms.
WB blocked with milk, 1AB overnight incubation at 4*C.
Please see image above.